Apoptotic effects of Staphylococcus aureus alpha toxin on DU145 cancereous cells

Prostate cancer is one of the most common cancers among men, which may also affect their fertility. Usually, prostate cancer grows slowly and is initially limited to the prostate gland, but if it grows, it spreads and affects other organs as well. While some prostate cancers are benign and can be cured with minimal treatment, others are malignant and can spread quickly. In this study, the apoptotic effects of Staphylococcus aureus alpha toxin as a recombinant vector on DU145 prostate cancer cells have been investigated. After culture, the cells were transfected with empty vector and recombinant vector containing Staphylococcus aureus alpha toxin gene using Lipofectamine.After 72 hours, the expression level of apoptotic genes including P53, BCL2 and BAX in normal cells, cells with empty vector and cells with recombinant vector were investigated by QRT-PCR. The findings showed that the expression level of the mentioned apoptotic genes in cells transfected with the recombinant vector containing Staphylococcus aureus alpha toxin gene had changed significantly and apoptosis had occurred in these cells. It seems that Staphylococcus aureus alpha toxin has anti-apoptotic and anti-cancer properties on DU145 prostate cancer cells.